Sub-Micro cells retain the exterior dimensions of a standard cell and are designed for the measurement of very small samples, with volumes ranging from 10μl to 160μl. The entrance to the sample compartment is hemispherical, being designed without sharp corners to eliminate potential loss of sample due to capillary action. The amount of sample required to fill the sample chamber is reduced to an absolute minimum, typically fifteen to twenty per cent greater than the absolute sample chamber volume. The sample compartment in the Sub-Micro range of cells optimizes the alignment of the sample for maximum sensitivity in the spectrophotometer. The sample is easily inserted into and retrieved from the cell, by careful use of a pipette. The correct 'Z' dimension needs to be selected for this range, typically 8.5 or 15mm. Cells are supplied with a PTFE cover and a tight sealing polypropylene cap.

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Choosing the correct "Z" dimension

The 'Z' dimension is the distance from the base of a cell to the center of the sample chamber window. The 'Z' dimension is very important for small volume cells of any design, where the sample compartment cross section dimension is very small. The correct 'Z' dimension should be added to the part number for small volume cells.

How to determine 'Z' Dimension:
1) Cut a piece of paper 12mm x 100mm
2) Punch a hole with a pen 15mm from one end and 8.5mm from the other end
3) Put your instrument in %T at about 535nm.
4) Place the paper in the cell holder and see which hole transmits light through the hole.
5) If neither transmits light, cut another piece of paper and try other dimensions from the end of the paper.

Spectrophotometer	"z" dimension (mm)
Spectrecology	15
Agilent	15
Beckman	8.5
Bio-Rad	8.5
Eppendorf	8.5
GBC	15
Hewlett Packard	15
Hitachi	varies
Jasco	11
Ocean Optics	15
Perkin-Elmer	15
Pharmacia	15
Shimadzu	15
StellarNet	15
Thermo Spectronic	8.5 and 15
Turner	8.5
Varian	20
Avantes	15